Size Exclusion Chromatography

Size Exclusion Chromatography In size exclusion chromatography ( molecular or gel filtration or gel permeation chromatography), molecules, and particles are separated. Small molecules penetrate the pores of the stationary phase, but large molecules do not. Because small molecules must pass through an effectively larger volume, large molecules are eluted first. This technique is widely used […]

CHROMATOGRAPHY

Chromatography is a Greek word that means “separations of pigments, the colored substances”. This word was first time used by Russian chemist Mikhail Semenovich Tswett in 1906. Purpose Chromatography is a technique in which a mixture is converted into its components along with stationary phase by mobile phase. phases: Stationary phase: it may be solid […]